Biosecurity is one of the pillars of the Scientific Platform Pasteur-USP (SPPU). This concept is applied systematically in the SPPU through training and strict monitoring of rules and procedures. The purpose is to be zealous for the security of the professionals involved in the research activities, avoiding risks to human health and to the environment when handling chemical and biological agents.

All of the professionals working at SPPU follow a series of rules established by the Internal Biosecurity Committee, such as: be covered adequately by vaccines; consult with a labor medicine doctor and receive a health clearance certificate; take periodical medical exams; follow the procedures lined out in the Biosecurity Manual; and take part in training courses offered by the Committee. Simulations are held, periodically, to check if the concepts taught in the courses were internalized by the professionals.

These actions seek to consolidate a biosecurity culture, so that researchers and technicians are able to identify and give notice about risks, and to know how to proceed in the event of an accident.

Four of the 17 laboratories of the platform are at biosecurity level 3 (NB3), where the studies focus on micro-organisms that transmit potentially lethal diseases. The facilities of these laboratories consist, individually, of pressure chambers and air filters to ensure the control of pathogens, and access to them is controlled. Only professionals who have been trained to deal with this level of control can work in these laboratories.

Furthermore, access to the platform is generally controlled by biometrics.